Journal: Research and Practice in Thrombosis and Haemostasis

Article Title: Evaluation of different commercial antibodies for their ability to detect human and mouse tissue factor by western blotting

doi: 10.1002/rth2.12363

Figure Lengend Snippet: Tissue factor expression in human pancreatic cancer cell lines. We used five human pancreatic cancer cell lines that express different levels of tissue factor (TF). A, The level of TF mRNA in the different cell lines was from the Genevestigator gene expression database. B, The level of TF protein in the different cell lines was determined by western blotting using an anti‐human TF polyclonal antibody from Cell Signaling Technologies 47769 (1:1000 dilution, final concentration 0.055 μg/mL). Glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) was detected using an anti‐human GAPDH antibody (1:1000 dilution, final concentration 0.2 μg/mL). Secondary antibodies were used at a 1:10 000 dilution in blocking buffer. A protein marker is shown on the left of the panel. C, The level of TF activity in the different cell lines was determined using a one stage clotting assay. Mean levels of TF mRNA and activity are shown

Article Snippet: The anti‐mouse TF polyclonal antibody from BioMedica Diagnostics detected a nonspecific band at ~48 kDa that was present in both the KPC2 Cas9 and KPC2 TF KO lines (Figure ).

Techniques: Expressing, Gene Expression, Western Blot, Concentration Assay, Blocking Assay, Marker, Activity Assay, Coagulation